Migrating a Test Tube Ant Colony to Your Formicarium Successfully
Here's a video showing how we migrate a test tube colony to our formicarium.
Some useful information:
Moving a test tube ant colony into a formicarium is a key step for any ant keeper looking to provide a better environment for their colony to grow. This process requires patience, care, and the right technique to avoid stressing the ants or losing members of the colony. In this guide, you will learn how to migrate your test tube colony smoothly, ensuring the ants settle comfortably into their new home. Make sure you have a lot of worker ants or this may harm the colony if moved too soon to a space too big.
Why Move Your Ant Colony from a Test Tube?
Test tubes serve as excellent starter homes for ant colonies, especially during the founding stage. They provide a simple, humid, and secure environment for the queen and her first workers. However, as the colony grows, the limited space and resources in a test tube become insufficient. Moving the colony to a formicarium offers several benefits:
More space for the colony to expand and build tunnels
Better visibility for observation and study
Improved ventilation and humidity control
Easier maintenance and feeding
Understanding when and how to migrate is crucial to avoid harming the colony.
Preparing for the Migration
Before starting the migration, preparation is key. Here are the steps to get ready:
Choose the Right Formicarium
Select a formicarium that suits the species and size of your colony. Consider:
Size: Large enough to accommodate growth but not too big to cause stress
Material: Acrylic or glass formicaria offer good visibility and durability
Design: Includes a nesting area and a foraging zone
Set Up the Formicarium Properly
Prepare the formicarium environment before introducing the ants:
Hydrate the nesting area to maintain humidity
Add substrate if required by the species
Ensure temperature matches the ants’ natural preferences
Place food and water sources nearby but outside the nest chamber
Gather Necessary Tools
Have these items ready for a smooth migration:
Soft brush or small paintbrush
Tweezers (preferably plastic or smooth metal)
Cotton balls or plugs
A small container for temporary holding if needed
Red light or dim lighting to reduce ant stress
Step-by-Step Migration Process
The migration should be calm and slow to avoid alarming the ants.
Step 1: Observe the Colony’s Behavior
Watch the ants for a day or two before migration. Look for signs they are ready to move, such as:
Workers exploring outside the test tube
The queen moving towards the opening
Ants carrying brood near the tube entrance
Step 2: Connect the Test Tube to the Formicarium
Create a direct path between the test tube and the formicarium:
Remove the cotton plug from the test tube end
Attach the test tube opening to the formicarium entrance using tape or a connector
Ensure the connection is secure and stable
Step 3: Encourage the Ants to Move
Ants naturally explore new spaces when conditions are right. To help them migrate:
Place food and water in the formicarium to attract workers
Apply heat to the formicarium in a gradient - but not too hot!
Avoid shaking or sudden movements
Step 4: Wait Patiently
Migration can take hours or even days. Do not force the ants out by shaking or tapping the test tube. Instead, allow them to move at their own pace.
Step 5: Remove the Test Tube After Migration
Once most ants, including the queen and brood, have moved into the formicarium:
Gently detach the test tube
Clean and store it for future use or discard safely
Tips for a Successful Migration
Maintain humidity in both the test tube and formicarium to keep ants comfortable
Use red light during observation since ants cannot see red light well, reducing disturbance
Avoid sudden temperature changes that can stress the colony
Do not feed immediately after migration; wait until ants settle to avoid attracting pests
Monitor the colony daily for signs of stress or escape attempts
Common Challenges and How to Overcome Them
Ants Refuse to Move
If ants stay inside the test tube:
Check if the formicarium is too dry or too bright
Try placing a small piece of cotton soaked in sugar water inside the formicarium
Wait longer; some colonies take more time to explore
Queen Does Not Move
The queen is critical. If she stays behind:
Do not force her out; this can cause injury or death
Ensure the test tube is connected properly and the environment is inviting
Sometimes, gently tapping the test tube near the opening can encourage movement
Ants Escape During Migration
To prevent escapes:
Work in a contained area with barriers
Use a shallow tray or container to catch any escapees
Close all openings except the migration path
After Migration Care
Once the colony is settled:
Keep the formicarium clean and free of mold
Provide regular food and water
Maintain stable temperature and humidity
Observe colony growth and behavior
Final Thoughts
Here's a video showing how we migrate a test tube colony to our formicarium.

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